Open AccessFgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat
Author(s) -
ZHANG DAJUN,
FAN FEIYU,
YANG JINGRAN,
WANG XULI,
QIU DEWEN,
JIANG LINGHUO
Publication year - 2010
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2010.00626.x
Subject(s) - biology , wortmannin , phosphatidylinositol , tensin , virulence , phosphatase , kinase , saccharomyces cerevisiae , pten , microbiology and biotechnology , signal transduction , genetics , yeast , pi3k/akt/mtor pathway , gene , phosphorylation
SUMMARY Both mammalian tensin‐like phosphatase 1 [TEP1; also known as phosphatase deleted on chromosome 10 (PTEN) or mutated in multiple advanced cancer 1 (MMAC1)] and Saccharomyces cerevisiae ScTep1p are involved in the phosphatidylinositol pathway. In this study, we identified the Fusarium graminearum locus FGSG_04982.3 (named FgTEP1 ) as the functional homologue of ScTEP1 in the sensitivity of S. cerevisiae cells to wortmannin, the phosphatidylinositol‐3 kinase inhibitor. Deletion of FgTEP1 causes F. graminearum mycelial growth to become sensitive to lithium and reduces the production of conidia. Although conidia lacking FgTEP1 germinate normally, they show reduced germination efficiency in the presence of wortmannin. In addition, we showed that deletion of FgTEP1 reduces the virulence of F. graminearum on wheat. These results indicate that FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway in this plant fungal pathogen.