Open AccessGenetic abnormalities involved in t(12;21) TEL – AML1 acute lymphoblastic leukemia: Analysis by means of array‐based comparative genomic hybridization
Author(s) -
Tsuzuki Shinobu,
Karnan Sivasundaram,
Horibe Keizo,
Matsumoto Kimikazu,
Kato Koji,
Inukai Takeshi,
Goi Kumiko,
Sugita Kanji,
Nakazawa Shinpei,
Kasugai Yumiko,
Ueda Ryuzo,
Seto Masao
Publication year - 2007
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2007.00443.x
Subject(s) - comparative genomic hybridization , runx1 , biology , etv6 , fusion gene , leukemia , chromosomal translocation , genetics , gene , mutation , genome , cancer research , transcription factor
The TEL (ETV6)–AML1 (RUNX1) chimeric gene fusion is the most common genetic abnormality in childhood acute lymphoblastic leukemias. Evidence suggests that this chimeric gene fusion constitutes an initiating mutation that is necessary but insufficient for the development of leukemia. In a search for additional genetic events that could be linked to the development of leukemia, we applied a genome‐wide array‐comparative genomic hybridization technique to 24 TEL–AML1 leukemia samples and two cell lines. It was found that at least two chromosomal imbalances were involved in all samples. Recurrent regions of chromosomal imbalance (>10% of cases) and representative involved genes were gain of chromosomes 10 (17%) and 21q (25%; RUNX1 ) and loss of 12p13.2 (87%; TEL ), 9p21.3 (29%; p16INK4a/ARF ), 9p13.2 (25%; PAX5 ), 12q21.3 (25%; BTG1 ), 3p21 (21%; LIMD1 ), 6q21 (17%; AIM1 and BLIMP1 ), 4q31.23 (17%; NR3C2 ), 11q22‐q23 (13%; ATM ) and 19q13.11‐q13.12 (13%; PDCD5 ). Enforced expression of TEL and to a lesser extent BTG1 , both single genes known to be located in their respective minimum common region of loss, inhibited proliferation of the TEL–AML1 cell line Reh. Together, these findings suggest that some of the genes identified as lost by array‐comparative genomic hybridization may partly account for the development of leukemia. ( Cancer Sci 2007; 98: 698–706)