Open AccessExpression of Periostin, Homologous with an Insect Cell Adhesion Molecule, as a Prognostic Marker in Non‐small Cell Lung Cancers
Author(s) -
Sasaki Hidefumi,
Lo KinMing,
Chen Lan Bo,
Auclair Daniel,
Nakashima Yoshiaki,
Moriyama Satoru,
Fukai Ichiro,
Tarn Carmen,
Loda Massimo,
Fujii Yoshitaka
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb01174.x
Subject(s) - periostin , lung cancer , biology , cancer research , gene expression , cell adhesion molecule , pathology , in situ hybridization , real time polymerase chain reaction , gene , reverse transcription polymerase chain reaction , microbiology and biotechnology , medicine , immunology , extracellular matrix , genetics
We used our palindromic polymerase chain reaction (PCR)‐driven cDNA differential display technique to identify and isolate a gene, designated periostin , from cancer tissues and found it to be overexpressed in several human tumors. We attempted to determine the influence of periostin expression on clinical outcome in patients with non‐small cell lung cancer (NSCLC) by reverse transcriptase (RT)‐PCR analysis. Periostin gene was highly expressed at the tumor periphery of lung cancer tissue but not within the tumor by in situ RNA hybridization, suggesting that expression of periostin may be involved in the process of tumor invasion. Periostin transcripts were detected in 50 (49.0%) of the tumor samples, although some paired normal lung samples showed weak expression. There was no relationship between periostin gene expression and gender, N‐ or T‐status. The NSCLC patients with periostin expression had significantly poorer survival than the patients without periostin expression ( P =0.0338).