Open AccessA Novel Amplicon at 9p23‐24 in Squamous Cell Carcinoma of the Esophagus That Lies Proximal to GASC1 and Harbors NFIB
Author(s) -
Yang ZengQuan,
Imoto Issei,
Pimkhaokham Atiphan,
Shimada Yutaka,
Sasaki Kohsuke,
Oka Masaaki,
Inazawa Johji
Publication year - 2001
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.2001.tb01112.x
Subject(s) - amplicon , synteny , biology , locus (genetics) , gene , gene duplication , genetics , microbiology and biotechnology , chromosome , cancer research , polymerase chain reaction
The non‐random amplification of DNA at 9p23‐24 observed in various types of human cancers, including esophageal squamous cell carcinomas (ESCs), may reflect the locations of important tumor‐associated genes. Our previous studies using ESC cell lines defined an amplicon in this region and identified a novel gene, GASC1 , as a target of the amplification. Since different regions within the same chromosome arm are often involved in amplification in a syntenic or non‐syntenic manner, we characterized the amplicon at 9p23‐24 in 35 ESC cell lines (29 KYSE series and 6 YES series), and examined possible involvement of non‐syntenic amplifications at 9p23‐24 in 32 primary ESCs. Our results clearly indicated that two target regions for DNA amplification exist at 9p23‐24; the major amplicon contains GASC1 , and the minor one harbors a transcription factor, NFIB , centromeric to the GASC1 locus.