Open AccessHuman T‐cell Leukemia Virus Type 1 Can Infect a Wide Variety of Cells in Mice
Author(s) -
Feng Renqing,
Tanaka Masakazu,
Abe Hideaki,
Arashi Noriko,
Sun Binlian,
Uchida Kazuhiko,
Miwa Masanao
Publication year - 1999
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1999.tb00664.x
Subject(s) - provirus , biology , cd8 , virology , cell , leukemia , virus , microbiology and biotechnology , human t lymphotropic virus 1 , cell type , antigen , t cell , antibody , immunology , t cell leukemia , immune system , gene , genetics , genome
Analysis of human T‐cell leukemia virus type 1 (HTLV‐1)‐infected cell types and the interplay of these infected cells in vivo should provide valuable information to elucidate the pathogenesis of HTLV‐1‐associated diseases in humans and in animal models. In this study, HTLV‐1‐infected cell types were identified in HTLV‐1‐infected C3H/HeJ mice. Pan T, CD + 4 , CD8 + 8 , granulocyte and pan B cell fractions in the splenocytes of MT‐2 cell‐inoculated mice were sorted by use of their cell surface high‐density expression of CD 3e , CD 4 , CD 8 , Gr‐1 and B 220 antigens, respectively, with a fluorescence‐activated cell sorter. the pX sequence of HTLV‐1 provirus in the lysate of each fraction was amplified by polymerase chain reaction and detected by Southern hybridization. Interestingly, in addition to the CD + 4 cell fraction, the pX sequence was also found in CD8+ cell, B cell and granulocyte fractions. The broad cell spectrum of HTLV‐1 infection in mice is consistent with the situation in humans. Our findings indicate that HTLV‐1 receptor or coreceptor is widely distributed among different cell types in mice.