Open AccessSimultaneous Expression of T‐Cell and Myeloid Cell Phenotypes in Eight Newly Established HTLV‐I‐positive T‐Cell Lines
Author(s) -
Koizumi Shigeki,
Sugiura Makoto,
Zhang XianKui,
Yamashiro Katsushige,
Iwanaga Michiyo,
Imai Shosuke,
Osato Toyoro
Publication year - 1992
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1992.tb02002.x
Subject(s) - myeloid , myeloid leukemia , biology , cell culture , t cell , leukemia , cd33 , cell , antigen , microbiology and biotechnology , human t lymphotropic virus 1 , cd3 , virology , immunology , cancer research , t cell leukemia , cd8 , genetics , cd34 , immune system , stem cell
Eight cell lines were established from patients with adult T‐cell leukemia, and from normal adults, by cocultivation with human T‐cell leukemia virus type I(HTLV‐I)‐producer cell lines in the presence of interleukin‐2. All of these cell lines harbored HTLV‐I and showed T‐cell markers CD2, CD3 and CD4, hut not B‐cell markers. Unexpectedly, all eight cell lines expressed a myeloid marker CD13 and three of the eight lines also expressed another myeloid marker CD33. Dual staining showed the simultaneous expression of CD3 and CD13 on the same cells. Thus, evidence was obtained for the expression of myeloid antigens on HTLV‐I‐harboring T cells.