Open AccessTransformation of Animal Cells with Human T‐Cell Leukemia Virus Type II
Author(s) -
Miyamoto Kanji,
Tomita Noriko,
Hayashi Kazuhiko,
Akagi Tadaatsu
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02634.x
Subject(s) - cell culture , microbiology and biotechnology , hamster , biology , virus , immunofluorescence , virology , cytoplasm , extracellular , antigen , leukemia , cell , antibody , immunology , biochemistry , genetics
Human T‐cell leukemia virus type II (HTLV‐II) was tested for its ability to transform normal animal cells. The HTLV‐II‐infected human T‐cell line, HTLV‐IIA was lethally X‐irradiated and cocultivated with normal leukocytes of rabbit and crab‐eating monkey and spleen cells of hamster. The transformed cell lines, designated Ra‐IIA, Si‐IIA and Ham‐IIA, were established. These cell lines were shown to be infected with HTLV‐II by the polymerase chain reaction method combined with the digoxigenin‐enzyme‐linked immunosorbent assay method. These cell lines were examined for viral antigens by the indirect immunofluorescence method. Although the cytoplasma of over 90% of the cells of Si‐IIA cell line was brilliantly stained, Ra‐IIA and Ham‐IIA cells were not stained. Electron microscopy of cells of the Si‐IIA line revealed C‐type virus particles in the extracellular spaces.