Open AccessOligoclonal Immunoglobulin Gene Rearrangements in Philadelphia Chromosome‐positive Common Acute Lymphoblastic Leukemia
Author(s) -
Ikeda Takeshi,
Kita Kenkichi,
Kawakami Keiki,
Ohno Toshiyuki,
Seki Shigeki,
Nasu Kaori,
Ueda Takanori,
Honjo Tasuku,
Shirakawa Shigeru
Publication year - 1990
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1990.tb02573.x
Subject(s) - immunophenotyping , immunoglobulin heavy chain , biology , myeloid leukemia , southern blot , gene rearrangement , microbiology and biotechnology , antibody , immunoglobulin gene , acute lymphocytic leukemia , myeloid , immunoglobulin light chain , immunology , leukemia , gene , antigen , lymphoblastic leukemia , genetics
The occurrence of more than two rearranged bands of immunoglobulin heavy chain (IgH) genes in B precursor acute lymphoblastic leukemia (ALL) has recently been documented. To elucidate the nature of such leukemias, we studied 30 patients with common ALL, including 6 patients with Philadelphia chromosome (Ph 1 )‐positive ALL, by immunophenotyping and genotyping. In 10 of the 30, Southern blotting showed oligoclonal patterns of IgH gene arrangements, which were frequently detected in Ph 1 ‐positive ALL. In one patient of the 10, three rearranged bands of Ig k chain genes were detected. Ph 1 abnormality and co‐expression of myeloid associated antigens were found in 5 and 5 of the 10, respectively. Detection of multiple fragments of IgH genes would be suggestive of multipotent progenitor origin of these ALL.