Open AccessEpidermal Growth Factor Induces the Expression of Its Receptor Gene in Human Gastric Carcinoma Cell Line TMK‐1
Author(s) -
Yoshida Kazuhiro,
Takanashi Atsushi,
Kyo Eikai,
Ito Masanori,
Ito Hisao,
Niimoto Minoru,
Hattori Takao,
Tahara Eiichi
Publication year - 1989
Publication title -
japanese journal of cancer research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 0910-5050
DOI - 10.1111/j.1349-7006.1989.tb01708.x
Subject(s) - autocrine signalling , epidermal growth factor , biology , transforming growth factor , messenger rna , epidermal growth factor receptor , gene expression , tgf alpha , cell culture , cancer research , receptor , growth factor , gene , endocrinology , microbiology and biotechnology , medicine , biochemistry , genetics
To ascertain the possible autocrine pathway in the growth promotion of gastric carcinomas, a study was made on the effects of exogenous human epidermal growth factor (hEGF) on the expression of mRNA for EGF, transforming growth factor‐α (TGF‐α), EGF receptor, FOS and MYC genes by TMK‐1 cells. Exogenous hEGF increased FOS and MYC mRNA levels 30 min and 1 h after the treatment, respectively. TMK‐1 cells accumulated the mRNA for EGF receptor about 7‐ to 8‐fold by 3 h after treatment Expressions of mRNA for EGF and TGF‐α genes were detected, but the amounts of the mRNA of these genes in TMK‐1 cells were not altered after the treatment.