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Simple and specific detection of Bordetella holmesii by using a loop‐mediated isothermal amplification assay
Author(s) -
Otsuka Nao,
Yoshino Shuji,
Kawano Kimiko,
ToyoizumiAjisaka Hiromi,
Shibayama Keigo,
Kamachi Kazunari
Publication year - 2012
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2012.00465.x
Subject(s) - loop mediated isothermal amplification , bordetella pertussis , bordetella , microbiology and biotechnology , biology , virology , dna , bacteria , genetics
A loop‐mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay results were in complete agreement with the results of previously published real‐time PCR assay, indicating that the former is a powerful tool for the accurate diagnosis and surveillance of B. holmesii .