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Effects of non‐iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis
Author(s) -
Yukitake Hideharu,
Naito Mariko,
Sato Keiko,
Shoji Mikio,
Ohara Naoya,
Yoshimura Mamiko,
Sakai Eiko,
Nakayama Koji
Publication year - 2011
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2010.00299.x
Subject(s) - porphyromonas gingivalis , biology , heme , mutant , microbiology and biotechnology , gene , gene expression , heme oxygenase , periodontal pathogen , biochemistry , bacteria , enzyme , genetics
ABSTRACT The oral anaerobic bacterium Porphyromonas gingivalis, which is implicated as an important pathogen for chronic periodontitis, requires heme for its growth. Non‐iron metalloporphyrins, In‐PPIX and Ga‐PPIX, were examined for antibacterial effects on P. gingivalis . Both In‐PPIX and Ga‐PPIX caused retardation of P. gingivalis growth in a dose‐dependent fashion. Microarray and qPCR analyses revealed that In‐PPIX treatment upregulated the expression of several genes encoding proteins including ClpB and ClpC, which are members of the Clp (caseinolytic protease, Hsp100) family, and aRNR, aRNR‐activating protein and thioredoxin reductase, whereas In‐PPIX treatment had no effect on the expression of genes encoding proteins involved in heme uptake pathways, Hmu‐mediated, Iht‐mediated and Tlr‐mediated pathways. P. gingivalis ihtA and ihtB mutants were more resistant to In‐PPIX than was the wild‐type parent, whereas hmuR and tlr mutants did not show such resistance to In‐PPIX. The results suggest that In‐PPIX is incorporated by the Iht‐mediated heme uptake pathway and that it influences protein quality control and nucleotide metabolism and retards growth of P. gingivalis .