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Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor
Author(s) -
Morita Masatomo,
Ohnishi Makoto,
Arakawa Eiji,
Bhuiyan N.A.,
Nusrin Suraia,
Alam Munirul,
Siddique A.K.,
Qadri Firdausi,
Izumiya Hidemasa,
Nair G. Balakrish,
Watanabe Haruo
Publication year - 2008
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2008.00041.x
Subject(s) - vibrio cholerae , el tor , biology , cholera toxin , cholera , vibrionaceae , polymerase chain reaction , microbiology and biotechnology , virology , mutation , genetics , gene , bacteria
ABSTRACT A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.