Premium
Proteomic Characterization of Enterohemorrhagic Escherichia coli O157:H7 in the Oxidation‐Induced Viable but Non‐Culturable State
Author(s) -
Asakura Hiroshi,
Panutdaporn Nantika,
Kawamoto Keiko,
Igimi Shizunobu,
Yamamoto Shigeki,
Makino Souichi
Publication year - 2007
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2007.tb03969.x
Subject(s) - escherichia coli , microbiology and biotechnology , biology , bacterial outer membrane , strain (injury) , oxidative stress , membrane integrity , ribosomal protein , ribosome , bacteria , rna , biochemistry , gene , membrane , genetics , anatomy
Abstract Enterohemorrhagic Escherichia coli (EHEC) O157 strain F2, a food isolate of an outbreak, is resistant to oxidative stress, but has increased stress‐sensitivity after passage through mice. The stress‐sensitive variant of F2 (designated MP37) has decreased culturability, but retains membrane integrity under stress conditions, indicating that the cells enter a viable but non‐culturable (VBNC) state. Proteomic analyses revealed that MP37 in the VBNC state had decreased levels of some oxidation‐responsive factors (AhpCF, AceF), but it markedly increased levels of outer membrane protein W (OmpW). Because F2 expressed higher levels of some ribosome‐associated proteins (RaiA, S6, Bcp) than MP37, the effect of animal passage on the induction of the VBNC state in the EHEC O157 cells might be due to ribosomal activity.