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High Efficiency Cross‐Reactive Monoclonal Antibody Production by Oral Immunization with Recombinant Norwalk Virus‐Like Particles
Author(s) -
Tanaka Tomoyuki,
Kitamoto Noritoshi,
Jiang Xi,
Estes Mary K.
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03864.x
Subject(s) - norwalk virus , virology , monoclonal antibody , immunization , biology , virus like particle , recombinant dna , virus , antibody , antigen , immunoglobulin m , immunoglobulin a , adjuvant , microbiology and biotechnology , immunoglobulin g , immunology , norovirus , biochemistry , gene
Abstract Monoclonal antibodies (MAbs) are important for in‐depth antigenic characterization and diagnosis of infections with human caliciviruses that cause almost all outbreaks of nonbacterial gastroenteritis. We compared different routes of immunization with nonreplicating virus‐like particles (VLPs) from recombinant Norwalk virus (rNV) and recombinant Mexico virus (rMX) administered to BALB/c mice to determine the efficiency of hybridoma production. Oral immunization with VLPs without adjuvant resulted in high yields of MAb‐secreting hybridomas (90%) to these VLPs of IgG (61%), IgM (29%) and IgA (10%) isotypes. Fusions with mesenteric lymph node lymphocytes yielded MAbs of various subclasses including IgG2a, IgG3, IgM and IgA. These results suggest that an immunization route that mimics the natural route of viral infection pathway may facilitate MAb technology by increasing the yields of antibody secreting hybridoma cells.