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Efficacy of Intracerebral Immunization against Pseudorabies Virus in Mice
Author(s) -
Shin JaeHo,
Sakoda Yoshihiro,
Kim Jae Hoon,
Tanaka Tomohisa,
Kida Hiroshi,
Kimura Takashi,
Ochiai Kenji,
Umemura Takashi
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03849.x
Subject(s) - pseudorabies , immunization , virology , virus , intramuscular injection , subcutaneous injection , titer , immune system , antibody , immunology , biology , medicine , bioinformatics
To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with formalin‐inactivated pseudorabies virus (PRV) by either subcutaneous (SC) or IC injection, and then 10 6 plaque‐forming units of PRV were introduced into the hindleg of the immunized or non‐immunized mice by intramuscular injection. The antibody titer in serum was elevated and boosted by additional immunization via both the SC and IC routes, but was higher after IC immunization. Intracerebrally immunized mice were completely protected from mortality and neurological signs, whereas all the non‐immunized and 80% of the subcutaneously immunized mice died after developing neurological signs. In mouse models, IC immunization is more effective at inducing a protective immune response against the transneural spread of PRV than SC immunization.