Premium
Development of a Real‐Time PCR Assay for Detection of gyrA Mutations Associated with Reduced Susceptibility to Ciprofloxacin in Salmonella enterica Serovar Typhi and Paratyphi A
Author(s) -
Morita Masatomo,
Ito Kenitiro,
Hirose Kenji,
Takahashi Hideyuki,
Shimuta Ken,
Terajima Jun,
Ohnishi Makoto,
Harada Makoto,
Matsuzaki Mitsuhiro,
Watanabe Haruo,
Izumiya Hidemasa
Publication year - 2006
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2006.tb03843.x
Subject(s) - dna gyrase , biology , salmonella enterica , ciprofloxacin , salmonella typhi , serotype , microbiology and biotechnology , salmonella , virology , bacteria , antibiotics , escherichia coli , genetics , gene
A real‐time PCR assay with the cycling probe method was used to detect mutations at codons 83 and 87 in the DNA gyrase A subunit encoded by gyrA in Salmonella enterica serovar Typhi and Paratyphi A clinical isolates. The susceptibility estimated from the results of the gyrA mutation assay was consistent with that identified by the culture method using an E‐test. This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin.