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Emergence and Serovar Transition of Vibrio parahaemolyticus Pandemic Strains Isolated during a Diarrhea Outbreak in Vietnam between 1997 and 1999
Author(s) -
Chowdhury Ashrafuzzaman,
Ishibashi Masanori,
Thiem Vu Dinh,
Tuyet Dinh Thi Ngoc,
Van Tung Tran,
Chien Bui Trong,
Seidlein Lorenz,
Canh Do Gia,
Clemens John,
Trach Dang Duc,
Nishibuchi Mitsuaki
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03513.x
Subject(s) - vibrio parahaemolyticus , serotype , biology , outbreak , pandemic , microbiology and biotechnology , virology , diarrhea , pulsed field gel electrophoresis , genotype , vibrionaceae , bacteria , gene , covid-19 , genetics , medicine , disease , pathology , infectious disease (medical specialty)
Abstract We characterized 523 Vibrio parahaemolyticus strains isolated during a survey of diarrhea patients in Khanh Hoa province, Vietnam between 1997 and 1999. Forty‐nine percent of the strains were judged to belong to the pandemic strains that emerged around 1996 and spread to many countries. These strains were positive in the GS‐PCR assay and carried the tdh gene. The ORF8 of the f237 phage genome, a possible marker of the pandemic clone, was absent in 10% of these strains. Eleven O:K serovars were detected among the pandemic strains and the strains representing all 11 serovars of pandemic strains were shown to be closely related regardless of the ORF8 genotype using arbitrarily primed PCR and pulsed field gel electrophoresis analyses. It was clear that a transition of major serovars occurred among the pandemic strains represented by the emergence of O3:K6 in 1997, O4:K68 in 1998, and O1:K25 in 1998 and 1999.