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Bacterial mRNA Purification by Magnetic Capture‐Hybridization Method
Author(s) -
Pang Xin,
Zhou Dongsheng,
Song Yajun,
Pei Decui,
Wang Jin,
Guo Zhaobiao,
Yang Ruifu
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03493.x
Subject(s) - messenger rna , biology , microbiology and biotechnology , ribosomal rna , 23s ribosomal rna , rna , gel electrophoresis , in situ hybridization , 16s ribosomal rna , bacteria , ribosome , biochemistry , gene , genetics
Abstract A magnetic capture‐hybridization method was developed for purification of bacterial messenger RNA (mRNA) from total RNA by removing 5S, 16S and 23S ribosomal RNAs (rRNA). The quality of mRNA was evaluated by A 260nm / A 280nm value, denatured gel electrophoresis and RT‐PCR. The results showed that highly purified and intact mRNA was obtained by this method. The magnetic capture‐hybridization is a rapid and simple method for bacterial mRNA purification and has promising potential for improving studies using bacterial mRNA.