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Cloning and Characterization of the fur Gene from Moraxella bovis
Author(s) -
Kakuda Tsutomu,
Oishi Daiki,
Tsubaki Shiro,
Takai Shinji
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03378.x
Subject(s) - biology , microbiology and biotechnology , cloning (programming) , gene , polymerase chain reaction , amplicon , southern blot , gene expression , western blot , biochemistry , computer science , programming language
A homologue of the ferric uptake regulator gene ( fur ) was isolated from Moraxella bovis by degenerate polymerase chain reaction and cloning. Fur protein of M. bovis exhibited 72.1% amino acid identity with Acinetobacter calcoaceticus Fur. Western blot analysis showed a decrease of Fur expression in response to sufficient‐iron conditions compared with deficient‐iron conditions. An electrophoretic mobility‐shift assay indicated that Fur protein binds to DNA fragments containing a putative Fur‐box derived from the upstream region of the M. bovis fur gene. Fur of M. bovis may regulate the expression of iron transport systems in response to iron limitation in the environment.