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The Complete DNA Sequence of the O Antigen Gene Region of Plesiomonas shigelloides Serotype O17 Which Is Identical to Shigella sonnei Form I Antigen
Author(s) -
Chida Toshio,
Okamura Noboru,
Ohtani Kiyoshi,
Yoshida Yoko,
Arakawa Eiji,
Watanabe Haruo
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02478.x
Subject(s) - orfs , shigella sonnei , biology , plesiomonas shigelloides , shigella flexneri , gene , shigella , plasmid , open reading frame , nucleic acid sequence , microbiology and biotechnology , genetics , escherichia coli , antigen , gene cluster , virology , peptide sequence , bacteria
We cloned and determined the sequence of a DNA region of approximately 15‐kb containing the cluster of genes required for O17 antigen expression in the Escherichia coli K‐12 strain from the chromosome of Plesiomonas shigelloides serotype O17:H2 strain. The sequencing analysis revealed that the minimum essential region of the P. shigelloides O17 antigen gene cluster had a size of approximately 11.5‐kb and contained 9 contiguous open reading frames (ORFs), which were almost identical to the corresponding ORFs of Shigella sonnei form I antigen gene region, except for IS 630 sequence, at the DNA as well as amino acid levels. The putative function of most of the ORFs could be determined on the basis of amino acid sequence similarities and characteristics. In addition, the G + C content of the P. shigelloides O17 antigen genes was lower than that of the chromosomal DNA of P. shigelloides and S. sonnei , suggesting that both P. shigelloides O17 and S. sonnei form I antigen genes had been derived from the same origin with a low G+C content.