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Cloning and Sequencing of the Vibrio parahaemolyticus fur Gene
Author(s) -
Yamamoto Shigeo,
Funahashi Tatsuya,
Ikai Hisato,
Shinoda Sumio
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01919.x
Subject(s) - vibrio parahaemolyticus , biology , escherichia coli , complementation , cloning (programming) , gene , polymerase chain reaction , sequence analysis , genetics , vibrio alginolyticus , microbiology and biotechnology , vibrio , bacteria , computer science , programming language , phenotype
Abstract A ferric uptake regulatory gene ( fur ) was cloned from Vibrio parahaemolyticus WP1 by a polymerase chain reaction‐based technique followed by functional complementation of a fur mutation in Escherichia coli. A sequence analysis showed that, at the amino acid level, the V. parahaemolyticus Fur protein is 81% identical with the Fur protein from E. coli and over 90% identical with those of the Vibrio species.