Cloning and Sequencing of the  Vibrio parahaemolyticus fur  Gene | Zendy

Yamamoto Shigeo | Zendy; Funahashi Tatsuya | Zendy; Ikai Hisato | Zendy; Shinoda Sumio | Zendy

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Cloning and Sequencing of the Vibrio parahaemolyticus fur Gene

Author(s) -

Yamamoto Shigeo,

Funahashi Tatsuya,

Ikai Hisato,

Shinoda Sumio

Publication year - 1997

Publication title -

microbiology and immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.664

H-Index - 70

eISSN - 1348-0421

pISSN - 0385-5600

DOI - 10.1111/j.1348-0421.1997.tb01919.x

Subject(s) - vibrio parahaemolyticus , biology , escherichia coli , complementation , cloning (programming) , gene , polymerase chain reaction , sequence analysis , genetics , vibrio alginolyticus , microbiology and biotechnology , vibrio , bacteria , computer science , programming language , phenotype

A ferric uptake regulatory gene ( fur ) was cloned from Vibrio parahaemolyticus WP1 by a polymerase chain reaction‐based technique followed by functional complementation of a fur mutation in Escherichia coli. A sequence analysis showed that, at the amino acid level, the V. parahaemolyticus Fur protein is 81% identical with the Fur protein from E. coli and over 90% identical with those of the Vibrio species.

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