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Detection of Human Immunodeficiency Virus‐1 Nucleic Acid on Inactivated Filter Paper Disks by Polymerase Chain Reaction and Microtiter Plate Assay
Author(s) -
Kunisada Takao,
Ando Shuji,
Saito Kunihiro,
Eshita Yuki,
Röder Wolfgang,
Kruse Michael,
Müller Werner E. G.,
Ushijima Hiroshi
Publication year - 1994
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1994.tb01835.x
Subject(s) - nucleic acid , biology , microtiter plate , polymerase chain reaction , microbiology and biotechnology , reverse transcriptase , dna , virology , virus , polymerase , filter paper , human immunodeficiency virus (hiv) , chromatography , biochemistry , chemistry , gene
Abstract Human immunodeficiency virus type 1 (HIV‐1) in cultured cells, peripheral blood samples and sera were adsorbed on filter paper disks and inactivated by heat or ethanol. Two procedures, the polymerase chain reaction (PCR) and microtiter plate assay (HMPA) were used to detect the nucleic acid. The sensitivity after different heat treatments with nested PCR for HIV‐1 DNA (or nested reverse transcription‐PCR for HIV‐1 RNA) was identical regardless of whether the samples were examined immediately or one month later. Inactivation by ethanol treatment resulted in a slight loss of sensitivity. The HMPA proved to be as reliable and specific as the conventional PCR technique. We conclude that the heat‐treated filter paper disk assay is suitable for identifying HIV nucleic acid in clinical samples sent to the laboratory from a distance, e.g. in an envelope.