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Enhancement of Antigen‐Presenting Function of Dendritic Cells with Culture Supernatants of Mouse Peritoneal Macrophages Stimulated with Certain Particulate Substances
Author(s) -
Miyazaki Hiroshi,
Ito Akira,
Osawa Toshiaki
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01468.x
Subject(s) - antigen , keyhole limpet hemocyanin , biology , microbiology and biotechnology , ovalbumin , hemocyanin , zymosan , t cell , immunology , biochemistry , immune system , in vitro
Abstract The production from murine resident peritoneal macrophages (M φ ) of a soluble factor, which was capable of enhancing the antigen‐presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M φ (M φ sup) were prepared by culturing peritoneal M φ with particles, i.e., zymosan A, latex, and sheep red blood cells (SRBC), or antigen‐antibody (Ag‐Ab) complexes such as keyhole limpet hemocyanin (KLH)‐anti‐KLH, ovalbumin (OVA)‐anti‐OVA, and SRBC‐anti‐SRBC complexes. When exposed to M φ sup during antigen pulsing DC induced a marked antigen‐specific T cell proliferation, relative to DC treated with the supernatants from M φ cultured without stimuli (control sup). On the other hand, M φ sup‐treated splenic M φ stimulated antigen‐specific T cell activation to almost the same extent as did splenic M φ treated with control sup. These results indicated that peritoneal M φ elaborated a soluble factor which preferentially enhanced the AP capacity of DC when stimulated with particles or Ag‐Ab complexes. Analytical gel filtration of M φ sup revealed that the factor had an apparent molecular weight of 27,000 daltons which was distinct from interleukin 1.