Structural Changes in the Nucleoid of  Bacillus subtilis  at Low Temperature | Zendy

Amako Kazunobu | Zendy; Takade Akemi | Zendy; Umeda Akiko | Zendy

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Structural Changes in the Nucleoid of Bacillus subtilis at Low Temperature

Author(s) -

Amako Kazunobu,

Takade Akemi,

Umeda Akiko

Publication year - 1986

Publication title -

microbiology and immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.664

H-Index - 70

eISSN - 1348-0421

pISSN - 0385-5600

DOI - 10.1111/j.1348-0421.1986.tb03013.x

Subject(s) - nucleoid , bacillus subtilis , cytoplasm , biology , bacteria , biophysics , ribosome , strain (injury) , biochemistry , microbiology and biotechnology , escherichia coli , rna , genetics , anatomy , gene

The external shape of the nucleoid of Bacillus subtilis strain w23 was examined with a new electron microscopic technique, the rapid freezing and substitution fixation method. The nucleoid of the log and stationary phase cells was recognized as an area devoid of ribosomes and widely dispersed in the cytoplasm, which was different from that observed in OsO 4 ‐fixed cells. If the bacteria were exposed to low temperatures (0 to 10 C), the nucleoid showed a highly concentrated shape in the middle of the cytoplasm. These structural changes were observed only when the bacteria were maintained in a high‐salt buffer. The results are discussed in relation to the membrane fluidity at low temperature.

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