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Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus
Author(s) -
Taniguchi Hatsumi,
Sato Kiyoshi,
Ogawa Midori,
Udou Takezo,
Mizuguchi Yasuo
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00684.x
Subject(s) - vibrio parahaemolyticus , lytic cycle , biology , microbiology and biotechnology , nucleic acid , dna , phagemid , bacteria , bacteriophage , virus , virology , gene , biochemistry , escherichia coli , genetics
Abstract Phage Vf33, a filamentous phage about 1,400 nm long and 7 nm wide, specific for Vibrio parahaemolyticus , was isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292 g/cm 3 . As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80 C and to treatment with diethylether, acetone or methanol but sensitive to chloroform. The nucleic acid of this phage is single‐stranded circular DNA 8.4 kb in size. The viral genome was converted to a double‐stranded replicative form in the host cell. Among the strains tested, only V. parahaemolyticus strains possessing K38 antigen was sensitive to the phage.