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Superinfection of HVJ Carrier HeLa Cells with Ultraviolet‐Irradiated Newcastle Disease Virus
Author(s) -
Iinuma Masao
Publication year - 1972
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1972.tb00627.x
Subject(s) - hela , newcastle disease , virus , virology , biology , chemistry , cell culture , microbiology and biotechnology , genetics
ABSTRACT The mechanism of enhancement of growth of partially ultraviolet (UV)‐inactivated Newcastle disease virus (NDV) in HeLa cells persistently infected with hemagglutinating virus of Japan (HVJ) (HeLa HvJ ) was investigated. HeLa HVJ cells and normal HeLa cells were inoculated with UV‐irracliated NDV under similar experimental conditions, and numbers of both infective centers and NDV‐antigen producing cells in each culture were counted. It was found that the percentage of HeLa HVJ cells which produced infectious NDV or NDV‐antigen during the first cycle of infection was approximately 1.5 times higher than that of normal HeLa cells. Survival curves of UV‐irradiated NDV showed that NDV appeared more resistant to UV when titrated in HeLa HVJ cells than in assay in normal HeLa cells. Possible explanations for this phenomenon were discussed