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A Case of Verrucous Carcinoma Associated with Human Papillomavirus Type 16 DNA
Author(s) -
Nishikawa Takeshi,
Kobayashi Hitoshi,
Shindoh Masanobu,
Yamashita Toshiharu,
Fujinaga Kei,
Ohkawara Akira
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb01325.x
Subject(s) - penectomy , verrucous carcinoma , pathology , dermis , polymerase chain reaction , nested polymerase chain reaction , nodule (geology) , biology , carcinoma , penile carcinoma , penis , microbiology and biotechnology , medicine , anatomy , penile cancer , gene , paleontology , biochemistry
We report here a case of verrucous carcinoma which occurred on the penis of a 75‐year‐old male. The nodule was first noted six months earlier and was whitish, cauliflower‐like, and 17 × 19 mm in size. The histopathological examination revealed hypertrophic epidermal proliferation with pale staining keratinocytes, extending into the deep dermis. Partial penectomy and inguinal lymph node dissection were done. No lymph node metastasis was recognized. DNA was isolated from the frozen tumor tissue and examined for the presence of human papillomavirus (HPV) 16, 18, and 33 DNA by the polymerase chain reaction (PCR), using common and specific primers. A 140 base pair (bp) band was amplified and finally determined to be the HPV16 sequence by dot‐blot hybridization.
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