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Immunohistochemical Localization of Ornithine Decarboxylase in Human Skin
Author(s) -
Verrando Patrick,
Juhlin Lenhart,
Lacour JeanPhilippe,
Ortonne JeanPaul
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03542.x
Subject(s) - ornithine decarboxylase , epidermis (zoology) , human skin , immunohistochemistry , basal cell carcinoma , staining , biology , sweat gland , melanoma , pathology , keratinocyte , microbiology and biotechnology , in vitro , basal cell , anatomy , cancer research , sweat , immunology , biochemistry , enzyme , medicine , paleontology , genetics
Abstract The localization of ornithine decarboxylase (ODC) in human skin and cultured keratinocytes was studied with an immuno‐histochemical method. ODC was found in the epidermis, hair follicles, sweat glands and errector muscles. Irritation induced by stripping or UV‐B irradiation did not change the staining pattern in the epidermis. In psoriasis, the staining was most marked at the tip of the epidermal rete ridges. In basal cell carcinoma, there was a homogeneous labelling of the tumor cells and, in squamous cell carcinoma, the labelling was strong but less homogeneous. Melanoma and dermal naevus also positively stained for ODC. Cultured human keratinocytes also showed ODC positive immunofluorescence. This technique detects the ODC antigen present rather than levels of ODC activity.