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Assessment of young yellow European eel Anguilla anguilla L. exposure to a CYP1A1 inducer by the quantification of increase in hepatic CYP1A1 mRNA using real‐time RT‐PCR
Author(s) -
Aubry E.,
Cag C.,
Lalanne Y.,
Mouchès C.
Publication year - 2007
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2007.01658.x
Subject(s) - inducer , biology , real time polymerase chain reaction , reverse transcription polymerase chain reaction , reverse transcriptase , messenger rna , anguillidae , polymerase chain reaction , biomarker , microbiology and biotechnology , medicine , endocrinology , fishery , biochemistry , fish <actinopterygii> , gene
Young European eels were exposed to β‐naphthoflavone (βNF). The CYP1A1 mRNA hepatic levels, measured by real‐time reverse transcription polymerase chain reaction, were correlated to the concentration of βNF and the time of exposure so that it can be used as a biomarker of European eel exposure to a CYP1A1 inducer.