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Identification and characterization of a novel splice variant of gonadotropin α subunit in the common carp Cyprinus carpio
Author(s) -
Wang Y.,
Hu W.,
Liu W.y.,
Wang Y.p.,
Zhu Z.y.
Publication year - 2007
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2007.01582.x
Subject(s) - biology , cyprinus , protein subunit , alternative splicing , common carp , carp , immunoprecipitation , splice , peptide sequence , microbiology and biotechnology , endocrinology , medicine , gene , genetics , messenger rna , fishery , fish <actinopterygii>
In this study, an alternative splicing transcript GtH‐α291 was identified by RT‐PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio . The analysis of GtH‐α291 amino acid sequence by the SignalP server predicted that the ‘missing segment’ might characterize as a signal peptide. In the secretion experiment, GtH‐α357 subunit could be secreted out of HeLa cells while GtH‐α291 could not, which confirmed the prediction. Co‐immunoprecipitation assay proved that GtH‐α291 subunit is able to interact with both FSH‐β and LH‐β as GtH‐α357 does. This is the first report concerning an alternative splicing transcript of a GtH α subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation.