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Isolation of freshwater and deep‐sea type opsin genes from the common Japanese conger
Author(s) -
Zhang H.,
Futami K.,
Yamada Y.,
Horie N.,
Okamura A.,
Utoh T.,
Mikawa N.,
Tanaka S.,
Okamoto N.,
Oka H. P.
Publication year - 2002
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2002.tb01568.x
Subject(s) - conger , biology , opsin , gene , rhodopsin , zoology , genetics , amino acid , ecology , biochemistry , retinal
Two rod‐like opsin genes, conf and cond , were isolated from the common Japanese conger Conger myriaster . The predicted proteins of conf and cond consisted of 354 and 350 amino acid (aa) residues respectively, and the aa identity was 87.4%. Phylogenetic analysis suggested that conf and cond are homologous to the freshwater type and the deep‐sea type rod opsin genes of freshwater eels. RT‐PCR analyses revealed that in both juveniles and adults cond is expressed in the retina and pineal complex, while conf is only expressed in the pineal complex at a low level.