Premium
Effect of genetic origin of the fish on in vitro proliferation of muscle myosatellite cells of rainbow trout
Author(s) -
Valente L. M. P.,
Paboeuf G.,
Fauconneau B.
Publication year - 2002
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.2002.tb00898.x
Subject(s) - rainbow trout , myogenesis , biology , in vitro , strain (injury) , fish <actinopterygii> , trout , anatomy , zoology , andrology , endocrinology , fishery , biochemistry , medicine
In two rainbow trout Oncorhynchus mykiss strains fed 5% of body mass for 1 week, no significant differences were observed between the two strains in the in vitro proliferation rate of myosatellite cells isolated from white axial muscle and studied for 8 days following the incorporation of 5‐bromo‐2′‐deoxyuridine. Nonetheless, the differentiation process and formation of myotubes started first in the strain displaying the fastest growth and the myotubes attained a more developed stage in these fish: more numerous and bigger myotubes. When the two strains were submitted to starvation for 1 week, the fast growing strain showed a faster response to the culture medium and a significantly higher proliferation rate than the slow growing one.