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Partial separation of the pituitary proteins of Labeo umbratus , Smith (mudfish)
Author(s) -
Hattingh J.,
Toit P. J.
Publication year - 1973
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1973.tb04429.x
Subject(s) - sephadex , biology , chromatography , glycoprotein , fraction (chemistry) , molecular mass , pituitary gland , specific activity , endocrinology , electrophoresis , medicine , biochemistry , microbiology and biotechnology , chemistry , enzyme , hormone
L. umbratus pituitary glands were partially separated by means of preparative polyacry‐lamide electrophoresis and chromatography on DEAE‐cellulose and Sephadex G‐50. Eight fractions were obtaìned and it was found that fraction 1 contained potent adrenocorticotropic stimulating activity, with a molecular weight in the region of 6000. Fractions 3 and 4 contained potent gonadotropic and a lesser degree of exopthalmic producing activity, with molecular weights in the region of 14 000 to 20 000. Fraction 6 displayed pigeon‐crop stimulating activity, with a molecular weight above 30 000. Fractions 7 and 8 displayed M.S.H. and vasopressinoxytocic activity respectively, with a molecular weight in the region of 5500–6500. Fractions 2 and 5 could not be identified. The fractions seemed to be glycoproteins and the results are discussed in relation to previous findings.