Premium
The effect of chemical inhibition of matrix metalloproteinases on the size of experimentally induced apical periodontitis
Author(s) -
Tjäderhane L.,
Hotakainen T.,
Kinnunen S.,
Ahonen M.,
Salo T.
Publication year - 2007
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.0143-2885.2007.01223.x
Subject(s) - lesion , pulp necrosis , molar , dentistry , matrix metalloproteinase , medicine , periodontitis , necrosis , pulp (tooth) , periapical periodontitis , pathology
Abstract Aim To determine the effect of matrix metalloproteinase (MMP) inhibition on periapical lesion formation in a rat model. Methodology The pulp chambers of mandibular fist molars of adult SD rats were exposed to be infected by oral microbes. The experimental group was fed 20 mg kg −1 MMP‐inhibitor chemically modified tetracycline‐3 (CMT‐3) daily in an oral gavage and the controls were fed the vehicle. After 2 and 4 weeks, the mandibles ( n = 10 in both groups at both times) were radiographed, decalcified and subjected to histological analysis. Extension of necrosis in first molar distal root canals was measured from the histological sections, and periapical lesion sizes in the same roots were determined from radiographs and histological sections. Mann–Whitney U ‐test was used for the statistical analysis. Results There was a statistically significant difference in the extension of necrosis in root canals between 2 and 4 weeks in the control group ( P < 0.05), but not with MMP inhibition. Radiographically, MMP inhibition increased the periapical lesion size by 70% and 34% after 2 and 4 weeks respectively ( P < 0.05 in after 2 weeks). In histological measurements, lesion size increased with MMP inhibition by 26% and 8% after 2 and 4 weeks respectively. Conclusions MMP inhibition affects pulpal and periapical inflammation, increasing the rate of spreading of necrosis in root canals and the rate of periapical lesion formation.