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Phosphatidylcholine‐specific phospholipase C but not gamma interferon regulate gene expression and secretion of CC Chemokine Ligand‐2 (CCL‐2) by human astrocytes during infection by Toxoplasma gondii
Author(s) -
Durand François,
BrenierPinchart MariePierre,
Berger Francois,
Marche Patrice N.,
Grillot Renée,
Pelloux Hervé
Publication year - 2004
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.0141-9838.2004.00722.x
Subject(s) - toxoplasma gondii , biology , chemokine , secretion , toxoplasmosis , immunology , monocyte , interferon gamma , microbiology and biotechnology , immune system , endocrinology , antibody
SUMMARY We have used human astrocytoma‐derived cells to investigate the cellular responses of central nervous system cells to Toxoplasma gondii infection. At 24 h post inoculation, the secretion of CCL‐2 (or Monocyte Chemotactic Protein‐1) was augmented six‐fold over the control. This secretion was down‐regulated by D609, a specific inhibitor of phosphatidylcholine‐dependent phospholipase C (PC‐PLC), but not modulated by gamma interferon (IFN‐γ). Ribonuclease protection assay analyses showed significant down‐regulation of CCL‐2 mRNA production during infection by Toxoplasma gondii when cells were treated by D609. The mRNA levels of the seven other chemokines studied were not modified by D609. CCL‐2 seems to contribute to the cell recruitment during human cerebral reactivation of Toxoplasma gondii . Cellular production of this CC chemokine during toxoplasmosis may be regulated by a PC‐PLC‐dependent pathway.