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Validation of the protective Ostertagia ostertagi ES‐thiol antigens with different adjuvantia
Author(s) -
Geldhof P.,
Vercauteren I.,
Vercruysse J.,
Knox D. P.,
Van Den Broeck W.,
Claerebout E.
Publication year - 2004
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1111/j.0141-9838.2004.00681.x
Subject(s) - ostertagia ostertagi , biology , immunology , antigen , ostertagia , helminths
SUMMARY Intramuscular immunization of calves with an excretory–secretory antigen fraction enriched for cysteine proteinase activity (ES‐thiol) and QuilA as adjuvant induces a protective immune response against the abomasal nematode Ostertagia ostertagi . The objectives of the present study were to confirm the protective capacity of ES‐thiol in combination with QuilA, to test Al(OH) 3 as adjuvant for vaccination against O. ostertagi and to look for correlations between protection and immunological effector responses. Calves(seven animals/group) were vaccinated three times intramuscularly with 100 µg antigen and/or adjuvant (ES‐thiol with QuilA, ES‐thiol with Al(OH) 3 , QuilA alone and Al(OH) 3 alone) and subsequently challenged with a trickled oral infection of 25 000 infective larvae in total over 25 days. Faecal egg counts in the ES‐thiol QuilA group were reduced by 56% during the two‐month period of the trial compared to the QuilA control group ( P < 0·002). Calves immunized with ES‐thiol QuilA had significantly smaller adult worms ( P < 0·002) and less eggs/female worm ( P < 0·05) compared to the QuilA control group. No differences in egg output, worm counts or parameters of worm fitness were observed in the ES‐thiol Al(OH) 3 group compared to the Al(OH) 3 control group. Although the protective immune mechanism against O. ostertagi remains unknown, protection in the ES‐thiol QuilA group was associated with high levels of parasite‐specific antibodies in the abomasal mucosa.