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Differential expression analysis of G olgi apparatus proteomes in hepatocellular carcinomas and the surrounding liver tissues
Author(s) -
Yang Yaying,
Luo Yi,
Li Xiujun,
Yi Yongfen
Publication year - 2014
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/hepr.12151
Subject(s) - western blot , hepatocellular carcinoma , proteome , downregulation and upregulation , biology , microbiology and biotechnology , proteomics , liver cancer , apoptosis , pathology , cancer research , medicine , bioinformatics , biochemistry , gene
Aim Hepatocellular carcinoma ( HCC ) is the sixth most common malignancy worldwide. Liver is the largest human digestive gland with abundant G olgi apparatus involved in cell division, migration and apoptosis and others. Methods In the present study, G olgi apparatus of HCC and the surrounding liver tissues were isolated by sucrose density gradient centrifugation and identified by electron microscopy and enzymology methods. Using 2‐ D gel electrophoresis and mass spectrometry, 17 differentially expressed protein of G olgi apparatus in HCC and the surrounding liver tissue were screened and identified in the M ascot database. Results Of those differentially expressed proteins, six were upregulated and 11 were downregulated, some of them were related to the biological processes such as protein sorting, glycosylation, cell cycle regulation, transcription regulation and G olgi integrity. One protein, annexin A 5, was verified to be upregulated in HCC by western blot. Conclusion The differentially expressed proteins may provide new insight into HCC biology and potential diagnostic and therapeutic biomarkers.