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JMJD 2B/ KDM 4B inactivation in adipose tissues accelerates obesity and systemic metabolic abnormalities
Author(s) -
Kang Changkeun,
Saso Kayoko,
Ota Kazushige,
Kawazu Masahito,
Ueda Takeshi,
Okada Hitoshi
Publication year - 2018
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/gtc.12627
Subject(s) - biology , adipogenesis , endocrinology , medicine , adipose tissue , gene knockdown , obesity , epigenetics , regulator , steatosis , gene , genetics
Abstract Obesity is a serious global health issue; however, the roles of genetics and epigenetics in the onset and progression of obesity are still not completely understood. The aim of this study was to determine the role of Kdm4b, which belongs to a subfamily of histone demethylases, in adipogenesis and fat metabolism in vivo. We established conditional Kdm4b knockout mice. Inactivation of Kdm4b in adipocytes (K4b KO ) induced profound obesity in mice on a high fat diet ( HFD ). The HFD ‐fed K4b KO mice exhibited an increased volume of fat mass and higher expression levels of adipogenesis‐related genes. In contrast, the genes involved in energy expenditure and mitochondrial functions were down‐regulated. Supporting these findings, the energy expenditure of Kdm4b‐deficient cells was markedly decreased. In addition, progression of glucose intolerance and hepatic steatosis with hepatocellular damages was observed. These data indicate that Kdm4b is a critical regulator of systemic metabolism via enhancing energy expenditure in adipocytes.