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The corepressor activity of Alien is controlled by CREB ‐binding protein/p300
Author(s) -
Eckey Maren,
Kraft Florian,
Kob Robert,
Escher Niko,
Asim Mohammad,
Fischer Heike,
Fritsche Marie K.,
Melle Christian,
Baniahmad Aria
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12211
Subject(s) - corepressor , creb , creb binding protein , chemistry , microbiology and biotechnology , repressor , biology , genetics , gene , transcription factor
The regulation of gene repression by corepressors is a controlled process. Surface‐enhanced laser desorption ionization MS proteomic analysis and a yeast two‐hybrid screen showed independently that the corepressor Alien interacts with the CREB ‐binding protein ( CBP ) coactivator. This interaction was further confirmed by coimmunoprecipitation and glutathione S ‐transferase pull‐down experiments, suggesting that Alien interacts in vivo and in vitro with the histone acetyltransferase ( HAT ) coactivators CBP and its paralog p300. Acetylation detection experiments indicated that Alien is acetylated in vivo . Furthermore, Alien interacts with the central region of CBP/p300 containing the HAT domain and becomes acetylated in vitro . When an inhibitor of CBP /p300 HAT activity was employed, the Alien‐mediated silencing was enhanced. Thus, these findings suggest crosstalk between corepressors and coactivators, and indicate fine‐tuning of corepressor function by post‐translational modification through corepressor acetylation. Structured digital abstractp300 binds to Alien α by pull down ( View interaction ) Alien α physically interacts with CBP by two hybrid ( View interaction ) Alien α physically interacts with MLK2 by two hybrid ( View interaction ) p300 acetylates Alien α by acetylation assay ( View interaction ) Alien α physically interacts with NAP1 by two hybrid ( View interaction ) Alien α physically interacts with TAFI68 by two hybrid ( View interaction ) Alien α physically interacts with CBP by anti bait coimmunoprecipitation (View Interaction: 1 , 2 , 3 ) Alien α binds to CBP by pull down ( View interaction )