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Bursaphelenchus fungivorus from P inus pinaster bark in P ortugal
Author(s) -
Fonseca L.,
Cardoso J. M. S.,
MoronLopez J.,
Abrantes I.
Publication year - 2014
Publication title -
forest pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 49
eISSN - 1439-0329
pISSN - 1437-4781
DOI - 10.1111/efp.12077
Subject(s) - biology , phylogenetic tree , internal transcribed spacer , ribosomal dna , restriction fragment length polymorphism , bark (sound) , ribosomal rna , spacer dna , genetics , sequence analysis , botany , dna , polymerase chain reaction , gene , ecology
Summary Bursaphelenchus fungivorus is reported for the first time in P ortugal, identified as associated with P inus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer ( ITS ) regions of ribosomal DNA . Intraisolate genetic variability was detected among ITS sequences of the P ortuguese B . fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of B ursaphelenchus species, revealed that the P ortuguese B . fungivorus isolate clusters with other B . fungivorus isolates, forming a separate group close to B . seani highlighting a molecular proximity of these two species.