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Leptin promotes a proinflammatory lipid profile and induces inflammatory pathways in human SZ 95 sebocytes
Author(s) -
Törőcsik D.,
Kovács D.,
Camera E.,
Lovászi M.,
Cseri K.,
Nagy G.G.,
Molinaro R.,
Rühl R.,
Tax G.,
Szabó K.,
Picardo M.,
Kemény L.,
Zouboulis C.C.,
Remenyik É.
Publication year - 2014
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/bjd.13229
Subject(s) - proinflammatory cytokine , leptin , medicine , endocrinology , biology , lipid droplet , adipocyte , lipid metabolism , stat protein , western blot , inflammation , stat3 , chemistry , signal transduction , adipose tissue , biochemistry , gene , obesity
Summary Background Leptin, the adipocyte‐secreted hormone that regulates weight, is known to link lipid metabolism with inflammation in various cell types. However, its role in human sebocytes has not yet been investigated. Objectives The purpose of this study was to investigate the effects of leptin in human sebaceous gland biology. Methods Expression of the long form of the leptin receptor (Ob‐Rb) was detected by real‐time quantitative reverse transcriptase polymerase chain reaction ( qRT ‐PCR) and immunochemistry. Lipid analysis was by high‐performance thin‐layer chromatography, gas chromatography–mass spectrometry and time‐of‐flight mass spectrometer mass detection. Lipid bodies were visualized by BODIPY staining using fluorescent microscopy and measured by flow cytometry. Interleukin (IL)‐6 and IL‐8 mRNA levels were assessed by real‐time qRT ‐PCR and their release was evaluated by enzyme‐linked immunosorbent assay. Cyclooxygenase (COX)‐2 and 5‐lipooxygenase (LOX) protein expression and phosphorylation of p65 and signal transducer and activator of transcription (STAT)‐3 were determined by Western blot analysis. Results Expression of Ob‐Rb was detected in human sebaceous glands and in cultured human SZ 95 sebocytes. The treatment of SZ 95 sebocytes with leptin led to enlarged intracellular lipid bodies, increased ratios of unsaturated/saturated fatty acids and decreased vitamin E levels. Further supporting a proinflammatory role, leptin induced COX ‐2 and 5‐ LOX expression in SZ 95 sebocytes and augmented the production of IL ‐6 and IL ‐8 cytokines. On leptin treatment, the STAT ‐3 and nuclear factor‐κB pathways were activated, indicating that these known leptin signalling pathways are active in human sebocytes. Conclusions Our findings suggest that leptin signalling may be involved in the proinflammatory regulation of sebaceous lipid metabolism and the induction of inflammatory enzymes and cytokines.