Premium
Investigation of serotype distribution and resistance genes profile in group B Streptococcus isolated from pregnant women: a Chinese multicenter cohort study
Author(s) -
Yan Yuzhong,
Hu Hua,
Lu Tingyan,
Fan Huiqing,
Hu Yan,
Li Gang,
Zhang Xianhua,
Shi Yi,
Xia Rong
Publication year - 2016
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/apm.12570
Subject(s) - serotype , biology , levofloxacin , erythromycin , microbiology and biotechnology , penicillin , genotype , polymerase chain reaction , multiplex polymerase chain reaction , clindamycin , quinolone , antibiotic resistance , drug resistance , virology , gene , antibiotics , genetics
We surveyed the group B Streptococcus ( GBS ) strains isolated from four teaching hospitals during 1‐year period to investigate the current serotypes and antimicrobial resistance status of these strains. A total of 231 non‐duplicate colonizing GBS isolates were collected from pregnant women. Antimicrobial susceptibility of these isolates was tested by the disk diffusion method. Serotype was performed by a multiplex polymerase chain reaction ( PCR ) method. Analysis of the resistance mechanisms was performed by PCR amplification and DNA sequencing. Seven serotypes (Ia, Ib, II , III , V, VI , and VIII ) were identified, and the prevalence ranged from 0.9 to 35.9%. All isolates were susceptible to the penicillin, ceftriaxone, and vancomycin. The resistance of all the isolates to erythromycin, clindamycin, and levofloxacin was 61.5, 51.9, and 35.5%, respectively. The erythromycin resistance was mainly associated with the genes ermB and ermB‐mef(A/E) (69.8%). The most predominant phenotype was cMLSB (77.5%). Five gene panels, including gyrA , parC , parE , gyrA‐parC , and gyrA‐parC‐parE , were detected. The most predominant genotype was gyrA‐parC‐parE triple mutation (69.5%). The S81L in gyrA gene, S79Y mutation in par C gene, and H225Y mutation in parE gene were discovered. The isolates with serotype III , V, and Ia were the most important clone concerning the prevalence and resistance.