Premium
Different genetic alteration of A20 in a Sézary syndrome case with Vα2‐Jα22 T cell clone
Author(s) -
Zhou Lingling,
Zheng Haitao,
Huang Xin,
Zhu Lihua,
Wu Suijing,
Zeng Chengwu,
Yang Lijian,
Chen Shaohua,
Luo Gengxin,
Du Xin,
Li Yangqiu
Publication year - 2018
Publication title -
asia‐pacific journal of clinical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 29
eISSN - 1743-7563
pISSN - 1743-7555
DOI - 10.1111/ajco.12672
Subject(s) - single nucleotide polymorphism , biology , clone (java method) , t cell receptor , carcinogenesis , microbiology and biotechnology , genetics , gene , cancer research , t cell , genotype , immune system
Abstract Background The comprehensive genetic alterations underlying the pathogenesis of Sézary syndrome (SS) remains largely unknown. Previous studies showed that alterations of tumor necrosis factor‐α‐induced protein 3 gene ( TNFAIP3 ; A20 ) are frequent in SS. In this study, we characterized the mutation and polymorphisms of A20 in a case with SS and compared with the genetic feature of A20 in T‐cell acute lymphoblastic leukemia (T‐ALL). Methods Using a novel approach based on the combination of fine‐tiling array comparative genomic hybridization ( and ligation‐mediated polymerase chain reaction (LM‐PCR) to identify SS clone, the polymorphisms in the A20 gene (promoter, exons 2–9 [coding region] and 3′UTR) were detected by PCR and sequencing. Results The malignant SS clone was identified as TCR Vα2‐Jα22 rearrangement without deletion at the A20 loci (6q23‐27 region) in the SS case. Six polymorphisms were identified, all of them are belonging to single nucleotide polymorphisms (SNPs) that are recorded in genebank: rs5029924, rs5029937, rs2230926, rs582757 and rs77191406, while rs2307859 was not identified in the SS sample, which is found in all T‐ALL. The alteration pattern of A20 in this case seemed different from the T‐ALL samples, in contrast, it is similar to the alteration of A20 in samples from rheumatoid arthritis or systemic lupus erythematosus with poor clinical outcome and cancer developing. Conclusions The genetic alteration of A20 in the SS case was different from the T‐ALL samples and similar to the cases with refractory autoimmune disease and related to tumorigenesis. The findings lead to discuss whether such SNPs of A20 may link the refractory autoimmune inflammation and the tumorigenesis.