Open AccessProduction of 11α‐hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis
Author(s) -
FelpetoSantero Carmen,
Galán Beatriz,
García José Luis
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13735
Subject(s) - fermentation , biochemistry , rhizopus oryzae , biotransformation , sterol , chemistry , bacteria , enzyme , synthetic biology , biology , cholesterol , genetics , bioinformatics
In this work we have rationally designed new recombinant bacteria able to produce 11alpha‐hydroxylated steroids in a single fermentation step using sterols as feedstock. The key elements to achieve this goal were the hydroxylase system from R. oryzae and the actinobacteria Mycocilibacterium smegmatis .