Open AccessA molecular screening assay to identify Chlamydia trachomati s and distinguish new variants of C. trachomatis from wild‐type
Author(s) -
Xiu Leshan,
Li Yamei,
Zhang Chi,
Li Yizhun,
Zeng Yaling,
Wang Feng,
Peng Junping
Publication year - 2021
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/1751-7915.13724
Subject(s) - chlamydia trachomatis , 23s ribosomal rna , high resolution melt , biology , chlamydiaceae , plasmid , virology , pathogen , microbiology and biotechnology , gene , polymerase chain reaction , genetics , ribosome , rna
Since timely determination of nvCT is critical for restricting further infection spread in the population, we have developed a screening assay based on high‐resolution melting (HRM) that can detect C. trachomatis and identify nvCT. Our results showed that the HRM scanning assay is not only able to directly identify C. trachomatis in clinical specimens, but also to correctly differentiate nvCT with mutation C1514T, C1515T and G1523A from the wild‐type.