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KINETICS OF DNA‐INDUCED BREAKS BY REDUCTONE TREATMENT: IN VITRO AND IN VIVO STUDIES
Author(s) -
Leitão A. C.,
Mottat H. C.,
Gomes R. Alcantara
Publication year - 1981
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/0031-868x.00164-i1
Subject(s) - dna , in vivo , in vitro , chemistry , escherichia coli , dna damage , biochemistry , kinetics , dna synthesis , biology , genetics , gene , physics , quantum mechanics
Abstract— Reductone, a keto‐aldehyde that blocks repair of UV‐induced DNA damage also produces DNA breaks. These breaks are observed either in irradiated or unirradiated wild type and uvrA strains of Escherichia coli. DNA breakage has also been observed after in vitro treatment of T4 phage DNA. The results suggest that the breaking ability of reductone is a result of a direct attack on DNA.