The  Escherichia coli  orthologue of the  Salmonella ushB  gene ( ushB c  ) produces neither UDP‐sugar hydrolase activity nor detectable protein, but has an identical sequence to that of  Escherichia coli cdh | Zendy

Schroder Wayne | Zendy; Burger Michelle | Zendy; Edwards Catherine | Zendy; Douglas Meaghan | Zendy; Innes David | Zendy; Beacham Ifor R. | Zendy; Burns Dennis M. | Zendy

Open Access

The Escherichia coli orthologue of the Salmonella ushB gene ( ushB c ) produces neither UDP‐sugar hydrolase activity nor detectable protein, but has an identical sequence to that of Escherichia coli cdh

Author(s) -

Schroder Wayne,

Burger Michelle,

Edwards Catherine,

Douglas Meaghan,

Innes David,

Beacham Ifor R.,

Burns Dennis M.

Publication year - 2001

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1111/j.1574-6968.2001.tb10821.x

Subject(s) - escherichia coli , salmonella , hydrolase , gene , sugar , enterobacteriaceae , biology , escherichia , chemistry , biochemistry , microbiology and biotechnology , bacteria , enzyme , genetics

Salmonella ushB , which encodes a membrane‐bound UDP‐sugar hydrolase, has an Escherichia coli orthologue ( ushB c ) which does not detectably produce this activity. In this report, we show that ushB c does not produce any detectable protein either, despite being transcribed normally. Remarkably, ushB c is shown to have 100% sequence identity with E. coli cdh , previously characterised as encoding an active CDP‐diglyceride hydrolase, an apparent contradiction with implications regarding enzyme evolution. We suggest that a useful gene designation is cdh ( ushB c ) rather than either ushB c or cdh , alone.

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