A comparison of the different methods available for determining BCG‐macrophage interactions in vitro, including a new method of colony counting in broth | Zendy

Fazal N. | Zendy; Bartlett R. | Zendy; Lammas D.A. | Zendy; Kumararatne D.S. | Zendy

Open Access

A comparison of the different methods available for determining BCG‐macrophage interactions in vitro, including a new method of colony counting in broth

Author(s) -

Fazal N.,

Bartlett R.,

Lammas D.A.,

Kumararatne D.S.

Publication year - 1992

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1111/j.1574-6968.1992.tb05921.x

Subject(s) - colony forming unit , uridine , macrophage , agar , enumeration , labelling , in vitro , glycerol , isotope dilution , microbiology and biotechnology , cell counting , monocyte , biology , uracil , chromatography , chemistry , biochemistry , bacteria , immunology , cell , mass spectrometry , rna , mathematics , cell cycle , dna , genetics , combinatorics , gene

Different methods of determining BCG viability based on colony forming unit (CFU) counting and radio‐isotope labelling were comparatively assessed. These included radio‐isotope labelling with [ 3 H]uracil, [ 3 H]uridine, [ 3 H]glycerol, and CFU counting, by both agar plate dilution, and microcolony counting in broth. The sensitivity ranges of the different techniques were determined in both macrophage‐free and macrophage‐treated systems and used to assess the anti‐mycobacterial potential of human monocyte‐derived macrophages following BCG infection.

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