Monoclonal antibody to a specific antigen from  Wolinella recta  ATCC 33238 | Zendy

Kurihara Hidemi | Zendy; Nishimura Fusanori | Zendy; Kokeguchi Susumu | Zendy; Kato Keijiro | Zendy; Dyke Thomas E. | Zendy; Murayama Yoji | Zendy

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Monoclonal antibody to a specific antigen from Wolinella recta ATCC 33238

Author(s) -

Kurihara Hidemi,

Nishimura Fusanori,

Kokeguchi Susumu,

Kato Keijiro,

Dyke Thomas E.,

Murayama Yoji

Publication year - 1991

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1111/j.1574-6968.1991.tb04835.x

Subject(s) - monoclonal antibody , antigen , antibody , microbiology and biotechnology , biology , western blot , subclass , epitope , bacteria , chemistry , biochemistry , immunology , genetics , gene

Four monoclonal antibodies (mAbs) to a specific antigen (150‐kDa protein antigen) isolated from Wolinella recta ATCC 33238 by acid extraction were obtained. The four antibodies were all of the IgG1 subclass and exhibited equally high specificity for the antigen. A battery of 14 strains of oral bacteria were screened for cross‐reactivity with each mAb by ELISA and Western blot analysis. Weak cross‐reactivity to some strains was observed which differed depending upon the mAb. Immuno‐electronmicroscopic studies were performed with a mAb which revealed that the 150‐kDa antigen was localized to the cell surface of W. recta ATCC 33238.

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