Synthesis of 3‐furylmethylpenicillin using an enzymatic procedure | Zendy

Ferrero Oscar | Zendy; Reglero Angel | Zendy; MartínVillacorta Javier | Zendy; MartínezBlanco Honorina | Zendy; Luengo JoséM. | Zendy

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Synthesis of 3‐furylmethylpenicillin using an enzymatic procedure

Author(s) -

Ferrero Oscar,

Reglero Angel,

MartínVillacorta Javier,

MartínezBlanco Honorina,

Luengo JoséM.

Publication year - 1991

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1111/j.1574-6968.1991.tb04378.x

Subject(s) - penicillium chrysogenum , pseudomonas putida , moiety , dna ligase , enzyme , chemistry , acyltransferase , biochemistry , stereochemistry , acyl coa

3‐Furylmethylpenicillin was synthesized in vitro from 3‐furylacetic acid, 6‐aminopenicillanic acid (6‐APA), CoA, ATP and Mg 2+ . The reaction was catalyzed in two steps by the enzymes phenylacetyl‐CoA ligase (PCL) from Pseudomonas putida and acyl‐CoA: 6‐APA acyltransferase (AT) from Penicillium chrysogenum . PCL catalyzes the activation of 3‐furylacetic acid to 3‐furylacetyl‐CoA (3‐F‐CoA) and AT acylates the amino group of 6‐APA with the 3‐furylacetyl moiety of 3‐F‐CoA, releasing CoA and 3‐furylmethylpenicillin.

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